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August 15, 2025Open Access

Characterization of mitochondrial membrane potential v1

Ashley W. Seifert Hemendra J. Vekaria Ebenezer Aryee

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Key Points

The protocol outlines how to assess mitochondrial membrane potential using JC-1 dye, critical for understanding cell health.
Characterization of mitochondrial potential is influenced by the behavior of JC-1 dye, an important fluorophore.
This study employs a specific protocol detailing the steps to evaluate membrane potential in fibroblasts using JC-1 dye.
Insights gained may highlight mechanisms related to cellular energy production and potential metabolic issues in fibroblasts.

Implication

This protocol details measuring mitochondrial membrane potential in fibroblasts, indicating potential cellular health.

Abstract

This protocol describes the procedure used by the Seifert Lab to characterize the membrane potential of mitochondria from fibroblasts using JC-1 dye.

Discussion

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Cite This Study

Seifert et al. (2025) studied this question.

synapsesocial.com/papers/68af5210ad7bf08b1ead9745 https://doi.org/10.17504/protocols.io.rm7vz9xxrgx1/v1

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