Assessment of two 1,2,3 triazole-related compound derivatives as having antiangiogenic property using cultured aorta ring and chorioallantonic membrane assay

Objectives: In this work, two 1,2,3 triazole related compounds (designated as H1 and H4) will be examined for their impact against new blood formation using an in vivo experimental model of forming a chorionic plexus in the chorioallantoic membrane (CAM) and an ex vivo sprouting microvessel model using rat aorta rings. Additionally, the mechanism behind their anti-angiogenic action will be explored. Inhibiting angiogenesis can be a valuable treatment avenue for cancer disorders because new blood vessel formation will facilitate tumor growth, invasion, and metastasis. Materials and Methods: Using the rat aortic ring (RAR) Assay, the rat aorta was divided into 1-mm sections as rings, were added to a prepared growth medium with specific additives to each well. Both synthesized 1,2,3 triazole related derivatives each alone was added at different dosages. The study proceeded with applying the CAM Assay where 10 mg/ml of each derivative was added and tested separately. A digital camera was used to take pictures of each CAM, and the blood vessel dimensions were measured digitally. Results: The RAR assay demonstrated that both new chemical entities inhibited angiogenesis in a concentration-effect dependent relationship. The CAM results demonstrated its significant blood vessel disruption action. Conclusion: This study showed that the derivatives of 1,2,3 triazoles significantly hinder the angiogenesis process, and could be classified as angiogenesis inhibitors.