Four New Coumarins From Calophyllum membranaceum With CYP Family 1 Enzymes Inhibitory Activity

Phytochemical investigation of the leaves and stems of Calophyllum membranaceum Gardn. et Champ. led to the isolation of four new coumarins, calomembolides G–J ( 1 – 4 ), along with three known coumarins, including calanolide E 2 ( 5 ), calanolide E 1 ( 6 ), and calopolyanolide B ( 7 ). Their structures were determined on the basis of extensive spectroscopic and spectrometric data analyses, and computational studies. Seven coumarins were tested for inhibitory effects on CYP family 1 enzymes. Compounds 4 and 6 exhibited significant inhibitory activity and selectivity toward CYP1B1 and CYP1A1 enzymes, respectively, showing inhibition rates of 79.11% ± 12.95% and 29.95% ± 8.09%. These compounds can provide a new material basis for the development of CYP1‐targeted antitumor drugs.