Abstract As an essential nutrient, vitamin is D closely linked to the prevention of various diseases, including osteoporosis, diabetes, and cardiovascular diseases. 25-Hydroxyvitamin D (25(OH)D) serves as the primary indicator for assessing vitamin D status, and its accurate measurement is critical for clinical diagnosis and treatment. Chemiluminescence immunoassay (CLIA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) are two commonly used detection methods, but methodological differences lead to variations in results, potentially influenced by factors such as age and gender. To investigate these differences, this study analyzed consistency and correlation, employing a Generative Fuzzy Inference System (GENFIS). Analysis of 138 samples revealed that LC-MS/MS yielded significantly higher 25(OH)D values than CLIA (p < 0.01, mean difference = 1.33 ± 3.71, 95% CI: -5.95-8.61), with strong linear correlation and consistency between methods (Cohen's Kappa = 0.8257, R²=0.9075, intraclass correlation coefficient (ICC) = 0.93). GENFIS analysis suggested that women aged 30–40 may amplify differences between the two methods. To validate this, 59 additional samples were analyzed, showing a relative risk (RR) of 3.18 (95% CI: 1.71–5.93, p < 0.05) for the experimental group (women aged 30–40) compared to the control group (other populations), preliminarily supporting the GENFIS inference. These findings partially elucidate the impact of age and gender on detection method variability, offering new insights for optimizing the clinical application of 25(OH)D testing.
Hong-kun et al. (Fri,) studied this question.