Photoprotective Properties of Macromycetes and Lichens Extracts Combinations

Fruiting bodies of the macromycetes Fomes fomentarius, Ganoderma lucidum, Hericium erinaceus, and Inonotus obliquus, as well as the biomass of the lichens Cladonia arbuscula, Evernia prunastri, Hypogymnia physodes, and Ramalina pollinaria, were extracted with methanol. Combinations of extracts from macromycetes and lichens were created with a total concentration of 200 μg/ml. Extracts from lichens were introduced in concentrations equal to the IC50 for keratinocyte culture (HACaT), and extracts from fungi were added until 200 μg/ml were reached in solution. SPF values, critical wavelength, UVA/UVB ratio, and DPPH inhibition percentage were determined for the created combinations of extracts. It has been established that the introduction of lichen extracts into solutions of macromycete extracts increases SPF by 2-4 times; reduces the critical wavelength by 5-15 nm; reduces the UVA/UVB value by 10-15%. Antiradical activity with respect to DPPH of both the original extracts and their combinations at concentrations of 200 μg/ml decreases almost 3 times.