Determination of Lethal Radiation and Ethyl Methane Sulphonate Doses for Mutagenesis in Green Gram (Vigna radiata (L.) Wilczek)

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Probit analysis reveals LD50 for gamma irradiation and EMS in green gram, suggesting improved mutation strategies.

The study found LD50 values for EMS at 0.4% across three green gram varieties, leading to effective mutagenesis.
Gamma irradiation LD50 ranged from 400 to 650 Gy, varying by green gram cultivar, indicating genotype sensitivity.
Increasing doses of EMS and radiation decreased germination and survival rates, reflecting their mutagenic effects.
These results support large-scale mutagenesis efforts, enabling enhanced genetic diversity in green gram cultivation.

Hlaing et al. (2025) studied this question.

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