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August 25, 2025Medical and Health Science JournalOpen Access

Evaluation of Antioxidant Activity of Methanol Extract of Neem Leaves (Azadirachta indica) Using DPPH, ABTS, and FRAP Methods

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Authors

NANoer AiniATAndri Tilaqza

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Overview

In vitro assessment reveals strong antioxidant potential in neem leaves, highlighting flavonoids and phenolics as key contributors.

Key Points

  • The methanol extract of neem leaves showed strong antioxidant activity in the DPPH assay with an IC₅₀ value of 54.91 ppm.
  • Phytochemical analysis confirmed the presence of flavonoids, phenolics, alkaloids, and terpenoids in the extract, indicating rich secondary metabolites.
  • Antioxidant activity assessment utilized DPPH, ABTS, and FRAP methods, with no IC₅₀ values determinable in ABTS and FRAP assays at concentrations up to 1000 ppm.
  • The findings suggest that neem leaves could provide a natural source of antioxidants, enhancing our understanding of their health benefits.

Cite This Study

Aini et al. (2025) studied this question.

synapsesocial.com/papers/68af5d63ad7bf08b1eae08d2https://doi.org/10.33086/mhsj.v9i1.7849
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  1. 1Evaluation of Antioxidant Activity of Methanol Extract of Neem Leaves (Azadirachta indica) Using DPPH, ABTS, and FRAP Methods2025 · 2 citations
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  5. 5Antioxidant, antimicrobial, and apoptosis-related activities of Azadirachta indica (Neem) leaf extract in MCF-7 and A549 cell lines2026 · 1 citations