Comparative evaluation of marginal adaptation of mineral trioxide aggregate and biodentine in combination with bioactive glass nanoparticles as a root-end filling material: A CLSM study

Aim: The aim of the study was to evaluate and compare the marginal adaptation of mineral trioxide aggregate (MTA) and biodentine (BD) in combination with bioactive glass nanoparticles (nBAG) as a root-end filling material under confocal laser scanning microscopy (CLSM). Methods: Forty extracted teeth were cleaned, autoclaved and decoronated. Cleaning, shaping, and obturation were done, and the access cavity was sealed coronally. After apicectomy, root-end cavity of 3 mm depth was done with ultrasonic tips. The samples were divided into 4 groups (n = 10) - Group 1: MTA, Group 2: BD, Group 3: MTA + nBAG, Group 4: BD + nBAG. All samples were kept in 100% humidity for a week, followed by immersion in 0.1% Rhodamine B dye for 48 h and then cut in 1 mm transversely under CLSM examination in ×10. Software Image J was used to measure the dye-stained area. Results: The combination of nBAG with BD has showed the least marginal gap area with the best marginal adaptation as compared to nBAG with MTA. Conclusion: The study concluded that the addition of nBAG to BD improves its marginal adaptation, a root-end filling material, as compared to MTA.