Creation of source for marking and mapping of frost resistance QTL in winter barley

Purpose. Creation the sets of recombinant-inbred lines for marking and mapping of frost resistance QTL in winter barley. Methods. Hybridization, artificial vernalization, growing plants under long-day conditions in phytotron light chambers and on a plot of land under natural daylight duration condition, DNA isolation with use CTAB-buffer, spectrophotometric determination of DNA concentration, polymerase chain reaction with direct primers, agarose and polyacrylamide gels electrophoresis, method of mathematical statistics chi-square test (χ2). Results. Using the SSD (single seed descent) method with maximum avoidance of selection, a set of 265 recombinant inbred lines F9 from the cross combination of the varieties ‘Khutorok’ / ‘Grabe’ was created, differing in the level of frost resistance, type of development and other traits. The creation of RIL from the crossing of the varieties ‘Akademichnyi’ / ‘Luran’ (170 lines F4) and ‘Tymofei’ / ‘Snihova Koroleva’ (145 lines F5) continues. The polymorphism of parental genotypes for the 14 microsatellite loci of chromosome 5H, including those localized in the region of the Fr-H1 and Fr-H2 key genes of the main frost resistance QTL. The allelic differences between parental varieties in several cross combinations were detected for a number of the studied microsatellite loci. The inheritance pattern of polymorphic microsatellite loci alleles in F1 hybrids from three cross combinations and individual plants from F2 populations ‘Akademichnyi’ / ‘Luran’ was investigated. Conclusions. The created RILs are suitable for use in barley genetic studies for identification, labeling, mapping of the main genes and QTL of qualitative and quantitative traits, primarily frost resistance. Polymorphic microsatellite loci were identified, which can be used for further analysis of recombinant-inbred lines, manipulation of genetic diversity, tracing inheritance and studying the effects of their alleles by the level of frost resistance and other traits of barley.