Transcriptomic and metabolomic findings highlight QseC's role in cell wall integrity and quorum sensing in Glaesserella parasuis.
Introduction Glaesserella parasuis ( G. parasuis ) causes agent Glässer’s disease in swine. This study investigated the mechanism of QseC in G. parasuis . Methods The study utilized transcriptomic and metabolomic sequencing techniques. The Δ qseC mutant was examined using transmission electron microscopy. Results Transmission electron microscopy revealed that Δ qseC mutant exhibited cell wall dissolution and cytoplasmic rarefaction, indicating membrane homeostasis disruption. Metabolomics analysis identified 819 metabolites, with 24/36 showing significant alterations in positive/negative ion modes. KEGG enrichment indicated abnormalities in amino acid synthesis and quorum sensing. Transcriptomic revealed 663 differentially expressed genes (DEGs), including upregulated membrane synthesis genes ( plsB and wecA ) and downregulated virulence factors ( hrpA and pilW ). Integrated analysis demonstrated that plsB and wecA formed association networks with methionine and prostaglandin metabolites. Discussion These results establish QseC’s global regulatory role in G. parasuis , providing insights for novel control strategies.
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Yan et al. (2025) studied this question.
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