The Era of Easy Creation of Eco-Friendly Pesticides: Algorithm of ‘Genetic Zipper’ Method in Action

The ‘genetic zipper’ method, based on CUAD (Contact Unmodified Antisense DNA) biotechnology, briefly CUADb or ‘genetic zipper’ method, represents a major breakthrough in eco-friendly pest control. This innovative approach is based on fundamentally new biological mechanism – DNA containment mechanism – and employs short, unmodified antisense DNA molecules to selectively degrade target rRNA in insect pests, disrupting protein synthesis and leading to high mortality rates. Demonstrating exceptional speed and precision, the method enables the design of effective and selective DNA pesticides for up to 10–15% of known insect pests in a single day. In this review, we highlight the simplicity and global applicability of this method using case studies involving 12 economically significant pest species, including hemipterans and one spider mite, from five continents. These oligonucleotide pesticides, generated via the DNAInsector web tool, are supposed to offer 80–90% efficacy against target pests within two weeks under laboratory conditions. Their application is primarily non-systemic, requiring direct contact, and they are environmentally safe, biodegradable, and highly specific, reducing risks to non-target organisms. The ‘genetic zipper’ method not only provides a powerful tool for researchers and practitioners but also opens a new era in pest management, where personalized, algorithm-driven pesticides can be easily created and applied for sustainable agriculture.