Penentuan Kadar Flavonoid Total dan Pembentukan Model Klasifikasi Serbuk Daun Singkong (Manihot esculenta Crantz) Berbeda Ketinggian Tempat Tanam

Cassava is a plant in the Euphorbiaceae family and is commonly found in Indonesia. One of the main secondary metabolites of cassava leaves is flavonoids. The secondary metabolite content of a plant is affected by several factors, including the height of the planting site. This research was conducted to determine differences in flavonoid content and to form a classification model of cassava leaf powder based on the height of the planting site. The UV-Vis spectrophotometer colorimetric method was used to measure the flavonoid content of cassava leaf powder, which was then analyzed statistically. The formation of the classification model was carried out using NIR spectroscopy and Chemometrics. The total flavonoid content of the sample from the highlands, medium plains, and lowlands was 13.599, 12.212, and 10.912 mg QE/gram powder, respectively. The One-Way ANOVA test showed that there is a significant difference in the average total flavonoid content of cassava leaf powder. The post hoc test showed that the data for each altitude had a significant difference from the other. The formation of LDA, SVM, and SIMCA classification chemometric models has 100% accuracy and is validated with 100% accuracy, so the LDA, SVM, and SIMCA models could categorize all samples in the valid category. Therefore, the selected and validated chemometric models are LDA, SVM, and SIMCA models.