Morphologic study of vessels, glia cells and neurons in the rectal wall in pediatric Hirschprung's disease

Purpose: To carry out histologic and IHC study of MCR vessels, glial and nerve cells in the intestinal wall, to compare the revealed changes with the character of clinical manifestations in Hirschsprung's disease Materials and Methods. The study included 10 cases with complaints of chronic constipation with various complications, depending on the duration of the disease. The cases were referred from the surgical department with a provisional diagnosis of Hirschprung's disease with a lifetime pathologoanatomical diagnosis using selective staining. Results. In spite of the generally accepted “gold standard” immunohistochemical diagnostics with antibodies with calretinin, in the practice of pathologists there are more and more frequent cases where the results of ganglion neurons examination are not enough to substantiate the diagnosis. Histologic and immunohistochemical study of microcirculatory vessels, glial and nerve cells in the intestinal wall, comparison of the revealed changes with the character of clinical manifestations on the example of 10 cases in patients of different age was carried out. Conclusion. Based on the data of morphological study, the lesion of microcirculatory vessels can cause the development of hypoxia, which should be considered as the main pathogenetic factor causing characteristic changes in the structures of the intestinal wall in Hirschsprung's disease.