Activity test of kitolod leaf ethanol extract gel (Isotoma longiflora) against acne bacteria

Background: Propionibacterium acnes is a bacterium that causes acne. Flavonoids, alkaloids, and saponins are believed to be present in kitolod leaves, potentially serving as antibacterials. This extract is formulated in a gel preparation to facilitate its use. Objective: Discover the antibacterial activity of kitolod leaf ethanol extract gel against Propionibacterium acnes bacteria, and the results on days 0, 7, and 14 were related to organoleptic, physical stability, homogeneity, pH, dispersion, and adhesion. Method: The maceration method was employed for extraction over a period of five days, using 96% ethanol as the solvent. The disc diffusion method was then used to test the antibacterial activity, measuring the diameter of the inhibition zone formed around the disc paper that had been inoculated with the test bacteria. Result: The result showed that the kitolod leaf ethanol extract gel has antibacterial activity against Propionibacterium acnes bacteria. FI (extract concentration of 25%), FII (extract concentration of 45%), and FIII (extract concentration of 65%) have an average diameter of each inhibition zone of 5.52 mm, 11.83 mm, and 17.67mm. All gel formulations on days 0, 7, and 14 were physically stable and met the requirements. Conclusion: The higher the concentration of kitolod (Isotoma logiflora) leaves extract gel, the higher the inhibition zone produced.