Development of sandwich ELISA to detect photobacterium damselae in aquaculture, highlighting improved specificity with two outer membrane proteins.
This study developed a dual-antibody sandwich ELISA detection method for Photobacterium damselae, an important pathogen in aquaculture, based on two outer membrane proteins of outer membrane protein C (OmpC) and β-barrel assembly machinery A (BamA) from the strain of P. damselae XP11. By optimizing the reaction concentrations of the capture antibody of rabbit anti-OmpC or anti-BamA and the HRP-labeled detection antibody of rabbit anti-BamA, it was found that using 1.0 μg/mL of rabbit anti-OmpC or 0.9 μg/mL of rabbit anti-BamA as capture antibodies, and 0.90 μg/mL of HRP-labeled rabbit anti-BamA as the detection antibody, could specifically detect different isolates of P. damselae. The detection limit of this method for the supernatant protein of P. damselae disrupted by ultrasound was 0.2 μg/mL. Repeatability tests showed that the coefficient of variation for detecting 25 strains of bacteria was below 9.1%. Compared with the OmpC-BamA sandwich ELISA detection method, the BamA–BamA combination exhibited better specificity. The results of this study provide an important reference for the rapid detection of P. damselae and other bacterial pathogens in aquaculture.
No takes yet. Share an insight, caveat, or question.
Chen et al. (2025) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: