Key result
A double-antigen sandwich ELISA for PCV2 antibodies demonstrated 94.38% sensitivity and 95.28% specificity compared with a commercial ELISA kit.
The newly developed double-antigen sandwich ELISA is a rapid and reliable method for detecting PCV2 antibodies in swine.
May support PCV2 serodiagnostics in swine; leaves open routine use pending field validation.
A double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) is described for detection of porcine circovirus 2 (PCV2) antibodies using the well-characterized recombinant PCV2 capsid protein. In a comparative test of 394 pig sera against an indirect immunofluorescence (IIF) test and a commercial ELISA kit (also based on the recombinant PCV2 capsid protein), the results showed that the diagnostic sensitivity, specificity, and accuracy of the assay were, respectively, 90.61, 94.02, and 91.62% compared with IIF and 94.38, 95.28, and 94.67% compared with the commercial ELISA kit. Assay of 12 PCV-free pigs over a 5-week period produced only PCV2-negative titers by all 3 methods. These results and the seroprofiles of 4 pig farms obtained by both the commercial ELISA kit and the double-antigen sandwich ELISA indicate that the sandwich ELISA is a reliable method for detection of antibodies to PCV2. Additionally, the method described here permits the use of undiluted test serum samples simultaneously loaded with horseradish peroxidase (HRP)-conjugated antigen into the test well, and the complete test procedure can be performed in less than 90 min. This double-antigen sandwich ELISA should be a useful tool to aid swine industry professionals in deciding the intervention strategies for the control of PCV2-associated diseases.
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Ge et al. (2012) studied Porcine circovirus 2 (PCV2) infection (n=394). Double-antigen sandwich ELISA vs. Indirect immunofluorescence (IIF) and commercial ELISA kit was evaluated on Diagnostic sensitivity, specificity, and accuracy. A double-antigen sandwich ELISA for PCV2 antibodies demonstrated 94.38% sensitivity and 95.28% specificity compared with a commercial ELISA kit.
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