Key result
An indirect ELISA using the PCV2 ORF2 antigenic domain demonstrated 93.57% sensitivity and 87.7% specificity for detecting PCV2 infection compared to an indirect immunofluorescent assay.
An I-ELISA using the ORF2 antigenic domain provides a specific, sensitive, and rapid method for detecting PCV2 infection in pigs.
Promising diagnostic performance in pigs; leaves open prospective validation before clinical or herd-level adoption.
BACKGROUND: The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we reported an indirect enzyme-linked immunosorbent assay (I-ELISA) by using an antigenic domain (113-147AA) of ORF2-encoded antigen, expressed in E. coli, for diagnosis of PCV infection. RESULTS: The ELISA was performed on 288 serum samples collected from different porcine herds and compared with an indirect immunofluorescent assay (IFA). In total, 262 of 288 samples were positive as indicated by both I-ELISA and IFA. The specificity and sensitivity of I-ELISA were 87.7% and 93.57%. CONCLUSIONS: This ELISA is suitable for detection and discrimination of PCV2 infection in both SPF and farm antisera.
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Sun et al. (2010) studied Porcine circovirus type 2 (PCV2) infection (n=288). Indirect enzyme-linked immunosorbent assay (I-ELISA) using GST-ORF2-E antigen vs. Indirect immunofluorescent assay (IFA) was evaluated on Diagnostic sensitivity and specificity. An indirect ELISA using the PCV2 ORF2 antigenic domain demonstrated 93.57% sensitivity and 87.7% specificity for detecting PCV2 infection compared to an indirect immunofluorescent assay.
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