Key result
A newly developed surface plasmon resonance imaging (SPRI) assay showed a strong positive correlation with conventional ELISA for detecting PCV2 antibodies in pig sera (r = 0.911, P<0.01).
Effect estimate: r = 0.911
p-value: p=<0.01
SPRI using a recombinant capsid protein is a viable, label-free, high-throughput alternative to ELISA for the serodiagnosis of PCV2 infection in pigs.
A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.
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Park et al. (2011) studied Porcine circovirus type 2 (PCV2) infection (n=70). Surface plasmon resonance imaging (SPRI) assay vs. Conventional ELISA was evaluated on Correlation between SPRI signal intensity and ELISA optical density values for detecting PCV2 antibodies (r = 0.911, p=<0.01). A newly developed surface plasmon resonance imaging (SPRI) assay showed a strong positive correlation with conventional ELISA for detecting PCV2 antibodies in pig sera (r = 0.911, P<0.01).
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