Implication of S-Lactoylglutathione in the Spontaneous Cysteine S-Glutathionylation and Lysine N-Lactoylation of Arabidopsis thaliana NAD-Dependent Glyceraldehyde 3P Dehydrogenase

In this study, the involvement of S,D-lactoylglutathione in the modification of the iso-form 1 of cytosolic glyceraldehyde 3P dehydrogenase (GAPC1) is examined. Incuba-tion of GAPC1 with S,D-lactoylglutathione resulted in inhibition of enzyme activity. The inhibitory effect was concentration-dependent and increased at alkaline pHs. Furthermore, inhibition of GAPC1 by S,D-lactoylglutathione was favored by oxidative conditions and reversed by reduction with dithiothreitol. Analyses of the S,D-lactoylglutathione-treated protein by nano LC-MS/MS revealed S-glutathionylation of its two Cys residues and N-lactoylation of six Lys residues. Pro-tein structure prediction showed that the double S-glutathionylation is accommodated by the GAPC1 catalytic pocket which likely explains enzyme inhibition. N-lactoylated sites overlap partially with previously reported N-acetylated sites at the surface of the GAPC1 tetramer. The efficiency of cytosolic glutaredoxin and thioredoxin isoforms was tested for reversing the S,D-lactoylglutathione-induced modification. In these as-says, recovery of GAPC1 activity after inhibition by S,D-lactoylglutathione treatment was used as indicator of efficiency. Results show that both types of redoxins were able to reverse inhibition. We propose a model describing the mechanisms involved in the two types of post-translational modification found on GAPC1 following exposure to S,D-lactoylglutathione. The possible involvement of these findings for the control over glycolytic metabolism is discussed.