Hsa_circ_0005325 Regulates the Proliferation, Apoptosis, Colony Formation, Migration, and Angiogenesis‐Promoting Behavior of Oral Squamous Cell Carcinoma Cells Through the miR‐433‐3p/HMGA2 Axis

Objective To explore the mechanism by which hsa_circ_0005325 affects the proliferation, apoptosis, colony formation, migration, and angiogenesis‐promoting behavior of oral squamous cell carcinoma cells through the miR‐433‐3p/HMGA2 axis. Material and Methods qRT‒PCR was used to measure the expression of hsa_circ_0005325 in SCC25 and CAL‐27 cells and normal human oral epithelial cells (HOK). SCC25 and CAL‐27 cells were cultured, and Cell Counting Kit‐8 (CCK‐8), cell apoptosis, plate colony formation, Transwell migration and a tube formation assays were used to detect changes in cell proliferation, apoptosis, colony formation, migration and angiogenesis, respectively. Results The expression of hsa_circ_0005325 was significantly increased in SCC25 and CAL‐27 cells. Compared with those in the sh‐NC group, the percentages of apoptotic SCC25 and CAL‐27 cells in the sh‐circ_0005325 group were significantly greater, and their proliferation, colony formation, migration and angiogenesis capacities were significantly lower ( p < 0.05). Moreover, the protein expression level of HMGA2 was significantly decreased, and the expression level of miR‐433‐3p was significantly increased in the sh‐circ_0005325 group versus the control group ( p < 0.05). Conclusion Hsa_circ_0005325 is highly expressed in SCC25 and CAL‐27 cells. Downregulation of hsa_circ_0005325 can inhibit the proliferation and invasion of SCC25 and CAL‐27 cells and promote their apoptosis.