Introduction: Bushenhuoxue formula is a traditional Chinese medicine formula with relatively safe clinical effects,but its mechanism in recurrent spontaneous abortion (RSA) is still unclear. Our present study used Network pharmacology an experimental validation to discuss how Bushenhuoxue formula improves prethrombotic state in RSA.Materials and methods: The active ingredients of Bushenhuoxue formula (Drug) were acquired from our previousstudy. The putative targets of ZYP relevant to AS were obtained from TCMSP, Swiss Target Prediction, STITCH,DisGeNET, and Gene Cards databases. Protein-protein interactions (PPI) network, Gene Ontology (GO), and KyotoEncyclopedia of Genes and Genomes (KEGG) analysis were conducted using the Cytoscape software. Furthermore,in vivo experiments were carried out for target validation in BALB/c mice, collecting placental tissue fromdifferent groups, the cell apoptosis by TUNEL assay; the pathology by HE staining; relative mRNA expression byqRT-PCR assay; relative protein expression by IHC and WB assay.Results: Animal experiments, compared with the NC group, the AT-III, PROG, HCG, APC and t-PA concentrationswere significantly depressed (P˂0.05, respectively), Apoptosis cell numbers were significantly up-regulatedwith PI3K/AKT/HIF-1α VEGF significantly depressing (P˂0.001, respectively). With Bushenhuoxue formula supplement, AT-III, PROG, HCG, APC and t-PA concentrations were significantly improved in RSA model mice; andimproved pathological changes and apoptosis cell number in placenta tissues (P˂0.05, respectively). However,with LY294002 supplement, the drug treatment effects were disappeared.Conclusion: Bushenhuoxue formula improves prethrombotic state in RSA via stimulating PI3K/AKT/HIF-1α/VEGFpathway in vivo.
Xuan et al. (Tue,) studied this question.
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