Mitochondria-ER contact site components regulate the formation and localization of specialized high-capacity mitochondria in the

Mitochondria-endoplasmic reticulum contact sites (MERCS) play crucial roles in mediating calcium signaling and lipid metabolism, and regulate mitochondrial morphology, function, and quality control. Recent studies have found that the C. elegans anchor cell (AC) harbors a specialized pool of high-capacity mitochondria that localize to the invasive front and are enriched with electron transport chain proteins to generate high ATP levels to fuel invasion. We conducted an RNAi screen of 59 MERCS-encoding components and identified over 30 required for high-capacity mitochondria formation. Our results suggest that MERCS may play a key role in the formation of specialized high-capacity mitochondria.