Production and purification of plasmid DNA with the Zymo Research ZymoPURE™ II Plasmid Gigaprep kit v1

This protocol is for production and purification of highest yields of highly pure and endotoxin-free plasmid DNA with the Zymo Research ZymoPURE™ II Plasmid Gigaprep Kit. The total yield can be up to 25 mg of DNA, we typically achieve between 6 and 12 mg in ≥3 mL elution volume. The concentration and purity is sufficient for electroporation in Aiptasia zygotes.