What question did this study set out to answer?

To establish a rapid, high-yield chromatography method using hydroxyapatite to purify plasmid DNA from bacterial cleared lysates.

November 1, 1978European Journal of BiochemistryOpen Access

Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography

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Authors

ACAlan ColmanSwinburne University of Technology MBMichael ByersGeisinger Medical Center SPSandy B. PrimroseTulane University

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Implication

Methodological study demonstrates rapid purification of high-purity plasmid DNA using hydroxyapatite chromatography, indicating an efficient alternative to conventional dye-centrifugation.

Key Points

To establish a rapid, high-yield chromatography method using hydroxyapatite to purify plasmid DNA from bacterial cleared lysates.
Applied bacterial cleared lysates to hydroxyapatite chromatography columns at room temperature in the presence of high concentrations of phosphate and urea.
Tested an optional heat-denaturation step to further minimize host chromosomal DNA contamination.
The chromatography procedure removed all detectable protein and RNA contaminants without altering plasmid conformation, leaving less than 0.5% chromosomal DNA.
Purified 5 mg of ColE1 plasmid DNA within 40 minutes, achieving greater purity and comparable yields relative to conventional dye-centrifugation.

Cite This Study

Colman et al. (1978) studied this question.

synapsesocial.com/papers/6a828fbd92c19701ea21dcb4 https://doi.org/10.1111/j.1432-1033.1978.tb20966.x

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Implication

Methodological study demonstrates rapid purification of high-purity plasmid DNA using hydroxyapatite chromatography, indicating an efficient alternative to conventional dye-centrifugation.

Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography

Discussion

Member takes

Implication

Key Points

Cite This Study

Rapid Purification of Plasmid DNAs by Hydroxyapatite Chromatography

Discussion

Member takes

Implication

Key Points

Cite This Study

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