Clean up of Index PCRs using SPRI Beads v1

Michael V. Westbury; Alba Ray-Iglesia; Vanssy Li; Deon de Jager; Eline D. Lorenzen

doi:10.17504/protocols.io.36wgqqkdxgk5/v1

May 8, 2025

Clean up of Index PCRs using SPRI Beads v1

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Key Points

Purified dna libraries were obtained with appropriate concentration measurements for downstream analysis.
The method used a 1.4:1 bead-to-dna ratio for effective size selection and clean-up of pcr products.
The protocol includes steps of bead binding, ethanol washes, and dna elution to ensure purity.
Quantification was performed using Qubit or other methods to assess the quality of purified dna libraries.

Implication

This protocol describes how to clean up pcr products with SPRI beads, ensuring high-quality dna libraries for analysis.

Abstract

This protocol describes the cleanup of index PCR products using SPRI bead-based size selection at a 1.4:1 bead-to-DNA ratio. The process includes bead binding, ethanol washes, DNA elution, and quantification using Qubit or other methods. The expected result is purified DNA libraries with concentration measurements for downstream analysis.

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Cite This Study

Westbury et al. (2025) studied this question.

synapsesocial.com/papers/68c189e09b7b07f3a0613a6f https://doi.org/10.17504/protocols.io.36wgqqkdxgk5/v1

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Clean up of Index PCRs using SPRI Beads v1

Key Points

Implication

Abstract

Discussion

Cite This Study

Clean up of Index PCRs using SPRI Beads v1

Key Points

Implication

Abstract

Discussion

Cite This Study

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