This protocol is used to prepare low-cost SPRI beads used for Illumina Library preparations. As part of the Hakai Institute Ocean Observing Program, from 0 m to near bottom (260 m), biomolecular samples have been collected weekly to genetically characterize plankton communities in the Northern Salish Sea since 2015. These SPRI beads have been used to clean up PCR products of 16S, 18S, COI, and 12S amplicons, and implemented as part of a standard procedure for eDNA analysis. This protocol is a modification from the following protocol: B. Faircloth and T. Glenn. Serapure v2.2. Ecol. and Evol. Biology, UCLA, November 19, 2011 Which was based on the following article:
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