In Vitro and In Vivo Evaluation of Wound Dressing Based on PCL Nanofibers Loaded With Linum usitatissimum Extract

In the present study, we fabricated nanofibers‐based wound dressing containing Linum usitatissimum (LOH) extract. The nanofibers were fabricated and characterized using electrospinning (applied voltage: 20 kV, feeding rate: 0.5 mL/min, and tip‐to‐collector distance: 10 cm). The SEM results indicated that the fabricated nanofibers have a straight and uniform morphology, and the diameter of PCL nanofibers loaded with 5%, 10%, and 15% of extract was 335 ± 45 nm, 438 ± 79 nm, and 471 ± 76 nm, respectively. Moreover, the nanofibers have porosity values in the range of 40%–55%. The in vitro biological evaluations revealed that adding the extract improved the biological performance (antioxidant, hemocompatibility, and cytocompatibility) of the pure PCL nanofibers. The in vivo study showed that the fabricated nanofibers improved the induced wound's healing process and enhanced the tissue's antioxidant state. These observations showed that combining electrospun nanofibers with LOH extract resulted in interactive and bioactive wound dressing nanofibers.