Protocol for assessing the effectiveness of lung preservation solutions in donation after circulatory death donors

Objective : to evaluate the effectiveness of lung preservation in donation after circulatory death (DCD) using a non-perfusion-based preservation method. Materials and methods . The study was conducted on eight healthy male Landrace pigs (weight range: 40–60 kg), divided into three groups based on the preservation solution used: Celsior group (n = 3), lungs preserved using a solution prepared according to the Celsior formulation.; NewSolution group (n = 3), lungs preserved using a custom-formulated solution developed in-house; NaCl group (n = 2), lungs preserved using saline (control group). Results . In the study groups (Celsior and NewSolution), the oxygenation index after reperfusion exceeded 350, while the control group (NaCl) exhibited an oxygenation index of less than 350. Conclusions . The method described for evaluating the effectiveness of new lung preservation solutions is technically simple and cost-effective, as it enables rapid experimentation with a sufficiently large number of observations. While this approach may not address all challenges in experimental transplantology, it provides a practical and efficient tool for preliminary screening of lung preservation strategies.