Amoxicillin loaded silver nanoparticles for antibacterial activity against some pathogenic bacteria associated with skin cancer

The emergence of antibiotic-resistant bacteria associated with skin cancer infections poses a significant therapeutic challenge. This study synthesized silver nanoparticles (AgNPs) and amoxicillin-loaded silver nanoparticles (ALNPs) using a cost-effective chemical reduction method. UV–Vis spectroscopy confirmed nanoparticle formation and stability, with ALNPs showing complete encapsulation of amoxicillin. Antibacterial activity was assessed against Staphylococcus aureus and Pseudomonas aeruginosa using the disc diffusion method with triplicate experiments (n=3). Minimal inhibitory concentration (MIC) results indicated that ALNPs were effective at lower concentrations than free amoxicillin. S. aureus was inhibited by ALNPs at 0.6 mg/mL compared to 5 mg/mL for free amoxicillin, while P. aeruginosa responded to ALNPs at 5 mg/mL and was resistant to free amoxicillin. Statistical analysis (ANOVA, p<0.05) confirmed significant improvement in antibacterial efficacy for ALNPs. These results suggest that nanoparticle-mediated delivery of amoxicillin enhances its antibacterial potency and may provide a promising strategy to overcome partial resistance in skin cancer-associated infections.