This review highlights diagnostic methods for fungal pathogens, indicating the role of sequencing and molecular techniques.
Mycology is a rapidly evolving field of science that has great potential to improve the understanding of fungi and their impact on the world. The review explores specific diagnostic methods employed in fungal diagnosis, tracing the evolution from traditional approaches to the more advanced and sophisticated techniques utilised in contemporary mycology. It emphasises the importance of integrating both conventional and innovative tools to enhance the early detection and accurate identification of fungal pathogens. Advances in sequencing technology and other molecular techniques are expanding the knowledge of fungal diversity and function. Synthetic biology is opening up new possibilities for using fungi as biotechnological tools. Many existing diagnostic methods are time-consuming, labour-intensive, and require specialised equipment and trained personnel. Therefore, there is a need for the development of diagnostic tests that are rapid, easy to use, and can be deployed in resource-limited settings. The future of medical mycology lies in integrating cutting-edge diagnostics with therapeutic innovation and a deeper understanding of host–pathogen interactions.
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Brown et al. (2025) studied this question.
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