Quantitative analysis of biofuel MET6 expression level in a treated Saccharomyces cerevisiae with garlic (Allium sativum L.) extract

Background: Methionine and cysteine are the common sulfur-containing amino acids integrated into proteins. However, methionine is the immediate initiating amino acid in biosynthesis of almost all eukaryotic proteins. Metabolism of methionine initiates with activating S-adenosylmethionine, where this cofactor plays numerous roles in transfer methyl group pathways. There are various biosynthetic paths of methionine, in fungi, the ultimate step of methionine biosynthesis is mediated by cobalamin-independent methionine synthase (MET6). Chemically produced methionine is widely used in various applications. However, the issue is the sulfur source, which is typically supplied as inorganic sulfate, which needs to be reduced to create methionine.Methods: Herein, testing the novel hypothesis that using natural resources such as garlic (Allium sativum), which is rich in organosulfur nitrogen-containing compounds, might enhance the biosynthesis of methionine. A quantitative analysis of MET6 mRNA was performed using qRT-PCR in all groups for treated S. cerevisiae (WT-BY4742) and met15Δ mutant strains with and without garlic extract.Results: In this study, according to the two-way ANOVA test, Met6 expression level was up-regulated significantly after 2 hours garlic exposure, and a significant increase in cell growth was observed of both mutant and wild-type strains compared with untreated control.Conclusion: It is concluded that garlic is a prominent natural source that may stimulate methionine biosynthesis, and this may be due to its organosulfur-containing compounds. Such plant sources could offer new natural approaches for methionine production and also support the prevention of certain diseases.