Evaluation of Bioremediation Competence of Indigenous Bacterial Strain Brevibacillus panacihumi KUESCCHK-5 isolated from Textile Effluent and its Effects on Vigna mungo

The textile industry generates highly contaminated wastewater containing harmful substances, posing significant environmental hazards. Bioremediation using natural organisms offers an effective treatment solution. This study involved the characterization of physico-chemical parameters of textile wastewater, followed by treatment using indigenous bacterial strain isolated from the effluent. The bacterial strain isolated from textile effluent identified as Brevibacillus panacihumi KUESCCHK-5 via 16S rRNA gene sequencing, was evaluated for its bioremediation potential under static conditions over five days. The strain achieved a decolorization rate of 99.38%-100% under optimal conditions (40°C, pH 9.5, peptone as a nitrogen source, glucose as a carbon source with a 4:1 carbon-to-nitrogen ratio). The treatment significantly reduced pollution parameters: BOD by 94.16%-99.11%, COD by 89.24%-98.83%, electrical conductivity by 79.06%-88.97%, fluoride by 89.08%-97.81%, phosphate by 91.38%-97.94%, turbidity by 89.76%-97.44% and sodium by 89.88%-97.65% at different effluent concentrations (Raw, 75%, 50% and 25% concentrations). ANOVA analysis indicated significant differences in pollutant levels across different effluent concentrations (p = 0.0436) and t-tests showed that the removal efficiency of physico-chemical parameters was significant at the 5% level but not at the 1% level. Additionally, heavy metals were reduced to below detectable limits and the treated effluent was non-toxic to Vigna mungo. These results indicate that Brevibacillus panacihumi is a promising, eco-friendly and cost-effective agent for treating textile effluent.