Spatial total RNA sequencing of formalin-fixed paraffin embedded tissue by spRandom-seq

Abstract Current sequencing-based spatial transcriptomic methods have been widely used to resolve gene expression; however, these methods are limited to fresh or fresh-frozen samples due to the low efficiency of oligo(dT) primers in degraded RNA samples. Here, we have developed a random primer-based spatial total RNA sequencing (spRandom-seq) technology for Formalin-fixed paraffin-embedded (FFPE) tissues by capturing full-length total RNAs with random primers. spRandom-seq providing a powerful spatial platform for clinical FFPE specimens.