This protocol illustrates histology and immunohistochemistry methods for cadaveric vagus nerves, suggesting effective imaging techniques.
This protocol describes the process to conduct histology (hematoxylin and eosin, H&E) and immunohistochemistry of embalmed human cadaveric vagus nerves, including grossing, tissue processing, embedding, slicing, H&E staining, immunohistochemistry (with antibodies against myelin basic protein, neurofilament, tyrosine hydroxylase, and calcitonin gene-related peptide), and imaging.
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Coleman et al. (2025) studied this question.
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