This protocol outlines procedures for protein extraction and sample preparation in larval zebrafish, indicating efficient mass spectrometry application.
This protocol describes the steps needed to prepare proteins from whole body larval zebrafish tissue for bottom-up shotgun proteomics via high resolution liquid chromatography mass spectrometry. Steps include solution preparation, sample collection, protein extraction, filter aided sample preparation, reduction, alkylation, enzymatic digestion via trypsin, and desalting steps using filter tips prepared in-house. This protocol results in a 1μg/μL solution of purified peptide samples.
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Abigail N. Henke (2023) studied this question.
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