Characterization of Bacillus Species Isolated from the Soil of Triveni Plant Assembly and Their Larvicidal Activity against Aedes aegypti

The Aedes aegypti mosquito is the major vector of dengue, chikungunya, and zika, posing significant public health risks that necessitate innovative management measures, such as introducing entomopathogenic bacteria. Bacteria from the soil of Triveni plant assembly (TPA), i.e., TPA (Neem, Peepal, and Bargad planted in very close vicinity), were isolated and evaluated for their toxicity to A. aegypti larvae. Five bacterial strains were chosen for molecular identification using 16S Rrna sequencing. The sequencing results indicated that the isolated bacterial strains are from distinct Bacillus species. All identified Bacillus spp. strains exhibited varying levels of larvicidal activity against A. aegypti larvae. Three different concentrations of bacteria were evaluated independently for larvicidal activity. Bacillus subtilis inaquosorum LGJT demonstrated the highest larvicidal efficacy (LC50 = 12.96 μL/mL). TPA soils, therefore, can be an efficient source of biological control agents against mosquito larvae..