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September 10, 2025Halalsphere

Evaluating PCR and ELISA for porcine detection in collagen-based products for halal authentication

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Authors

MSMuhamad Shirwan Abdullah SaniCBCamilla Dewanthy Putri BasukiRZRuzanna Zainal

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Overview

Comparative analysis of PCR and ELISA for porcine detection in collagen products, suggesting enhanced reliability for halal authentication.

Key Points

  • PCR identified porcine DNA in three out of nine collagen samples, highlighting its effectiveness in reliable detection.
  • ELISA detected porcine antigen in two samples, including one missed by PCR, demonstrating its complementary strengths.
  • However, both methods failed to detect porcine content in some cases, leading to a high false negative rate of 66.7%.
  • Integrating both PCR and ELISA enhances overall reliability in halal authentication, especially for various product types.

Cite This Study

Sani et al. (2025) studied this question.

synapsesocial.com/papers/68c1a40954b1d3bfb60de803https://doi.org/10.31436/hs.v5i2.130
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