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December 5, 2025Journal of Agricultural and Food Chemistry

Enhancing Food Transparency: PCR-HRM-Based Multiplex Assay for Simultaneous Detection of Four Animal Species in Food

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Authors

LCLong ChengDLDaofeng LiuXSXiao‐Mei Sha

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Overview

Multiplex assay identifies animal species in food, suggesting an effective tool against food adulteration.

Key Points

  • Detecting limit for DNA was as low as 0.05 ng/μL, ensuring reliable identification.
  • Multiplex assay accurately identifies porcine adulteration in food matrices at 1%.
  • High-resolution melting coupled with polymerase chain reaction aids in food species verification.
  • This method highlights the need for effective food authenticity protocols to combat fraud.

Cite This Study

Cheng et al. (2025) studied this question.

synapsesocial.com/papers/6940225c2d562116f28fc6a7https://doi.org/10.1021/acs.jafc.5c10963
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