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November 13, 2025FoodsOpen Access

Establishing a Detection Method Based on Multiplex PCR for Identification of Sheep Meat, Goat Meat and Common Adulterant Meats

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Authors

YYYanbing YangKQKai QuanHYHuiguo Yang

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Overview

This analysis identifies adulteration in sheep and goat meat using multiplex PCR, suggesting it can enhance meat quality control.

Key Points

  • The research aims to develop a multiplex PCR system for detecting adulteration in sheep and goat meat.
  • Designed species-specific primers targeting mitochondrial DNA sequences.
  • Optimized multiplex PCR conditions for specificity and reproducibility.
  • Evaluated practicality using simulated mixed samples and heat-treated products.
  • The system successfully identifies sheep meat, goat meat, and common adulterants.
  • It demonstrates high sensitivity for low DNA concentrations and adulteration ratios.
  • Robustness is confirmed as target components are detectable in heat-treated products.

Cite This Study

Yang et al. (2025) studied this question.

synapsesocial.com/papers/692523bbc0ce034ddc354b21https://doi.org/10.3390/foods14223875
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