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September 16, 2025FoodsOpen Access

Simultaneous Detection of Four Foodborne Pathogens in Raw Freshwater Fish Using High-Resolution Melting Analysis

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Authors

SSShan ShanHarbin Institute of TechnologyXTXiaoyu TongGuangxi UniversityWDWenyu DuChina Pharmaceutical University

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Implication

Multiplex PCR and high-resolution melting analysis detect foodborne pathogens in fish, suggesting enhanced food safety.

Key Points

  • The assay successfully detected four foodborne pathogens in raw fish, ensuring food safety.
  • Results showed perfect specificity for tested pathogens, with sensitivity rates ranging from 100% to 100%.
  • Detection limits for the PCR-HRM assay were between 0.02 to 0.1 ng/µL of genomic DNA.
  • The method efficiently identifies pathogens without significant interference from non-target strains.

Cite This Study

Shan et al. (2025) studied this question.

synapsesocial.com/papers/68d454c531b076d99fa5a235https://doi.org/10.3390/foods14183202
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