Synthesis and antiproliferative evaluation of novel triazolothiadiazine compounds

A novel series of triazolothiadiazines has been successfully synthesized with high efficiency through Knoevenagel condensation, utilizing thiocarbamoyl dihydrazine as the starting material. The structures of these compounds were characterized through 1 H-NMR, 13 C-NMR, high-resolution mass spectrometry. The MTT assay was utilized to evaluate the in vitro cytotoxicity of the synthesized compounds against four human cancer cell lines including SW620, A549, Hela, and MCF-7. Among the tested compounds, (Z)-3-methyl-6-phenyl-7-(3-(trifluoromethyl)benzylidene)-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine exhibited notably potent antiproliferative activity against all four human cancer cell lines.