Comparative Evaluation of Ceftriaxone Efficacy Against Escherichia coli: A Study of Three Commercial Brands Using Minimal Inhibitory Concentration

Objective: This study compares the efficacy of three commercially available ceftriaxone brands in Libya (from Tunisia, Spain, and India) against Escherichia coli using minimal inhibitory concentration (MIC) assays. Methods: A susceptible strain of E. coli was tested following CLSI protocols. MIC values were determined using serial two-fold dilutions of ceftriaxone in Mueller-Hinton broth. Experiments were conducted in triplicate under controlled laboratory conditions.Results: The Spanish and Indian brands exhibited lower MIC values (0.1172–0.2344 mcg/mL), indicating higher efficacy, while the Tunisian brand showed reduced efficacy with MIC values (2.7581 mcg/mL) exceeding standard benchmarks. Conclusions: These findings underscore the variability in the efficacy of ceftriaxone brands, emphasizing the need for stringent quality control and standardized testing.