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September 10, 2025Nano LettersOpen Access

Multiparametric Characterization of Individual Suspended Nanoparticles Using Confocal Fluorescence and Interferometric Scattering Microscopy with Microfluidic Confinement

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This research combines microscopy techniques for detailed analysis of LNPs, suggesting improved therapeutic efficacy.

The study successfully characterizes the size, mass, and payload of individual LNPs, enhancing understanding of therapeutic effects.
Key metrics include the simultaneous measurement of size, mass, and nucleic acid payload of LNPs in varying pH solvents.
The approach employs a commercially available confocal microscope and a convex lens instrument for effective characterization.
This method offers a simpler path for nanoparticle developers, potentially increasing the adoption of quantitative nanoparticle analysis.

Boateng et al. (2025) studied this question.

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