The Potential Hypoglycemic Activity of Gnetum latifolium Ethanolic Leaf Extract

The study aimed to evaluate the potential hypoglycemic activity of Gnetum latifolium ethanolic leaf extract. This investigation was initiated due to the limited research on the hypoglycemic properties of G. latifolium, utilizing ethanolic extraction methodology applied to the leaves. The extract was administered at three distinct concentrations corresponding to 25%, 50%, and 75% of the LD50, with metformin serving as the positive control and distilled water as the negative control group. A high-sugar diet protocol was employed to induce hyperglycemia in Swiss male albino mice. The main results demonstrated a significant dose-dependent decrease in blood glucose levels among treated mice, as measured through in vivo tail-tip blood sampling assays. Statistical analysis using one-way ANOVA was used to reveal significant differences which have a p-value of 0.74 between treatment groups. By establishing G. latifolium as a promising natural hypoglycemic agent, this study aims to open the door for further research into its clinical applications and therapeutic mechanisms.