Analysis of eluents for detection of organophosphorus compounds in milk by thin-layer chromatography

The aim of the study was to select optimal eluents for detecting the residual amount of organophosphorus compounds in milk using thin-layer chromatography. The research was conducted on the basis of the Department of Veterinary and Sanitary Expertise of St. Petersburg State Medical University. The research materials were solutions of malathion, chlorophos, diazinone and dimethoate. The detection of these substances in various solvent systems was carried out, followed by the calculation of the Rf mobility coefficients. The results were evaluated in the rays of UV light using UV cabinet UVK-HDi. As a result, the most sensitive eluent systems were identified: hexane–acetone 5:1 for diazinone and dimethoate, hexane–acetone 1:1 for chlorophos, and chloroform for malathion. The use of these systems makes it possible to isolate a substance and detect it using our proposed methodology.