Field Sampling of

We conducted surveys for the invasive drosophilid Zaprionus indianus in the eastern United States in 2023 and 2024. We found no latitudinal trends in Z. indianus abundance, and the northern boundary for Z. indianus was variable between years. Z. indianus was rare in central and northern Florida in the spring, was not present on early season berry crops in Virginia, and had a restricted temperate growing season compared to other drosophilids, providing further support for the species' yearly recolonization of temperate areas. Z. indianus was also observed on native fruits in a natural area in Virginia.