This protocol demonstrates effective dna extraction yields from whale skin samples, suggesting utility in forensic applications.
This protocol describes our latest protocol for extracting DNA from standard biopsy samples of whale skin. It uses a combination of phenol:chloroform to separate the DNA from other cellular material, and then Microcon filters to collect the DNA and then dissolve in the desired volume. This approach was motivated by original phenol:chloroform protocols described in Sambrook & Russell (2001), but modified based on organic extraction protocols used in the forensic sciences. From this protocol, we usually obtain ~250ng of DNA per milligram of starting tissue (a higher yield than that obtained from Qiagen columns, as well as phenol:chloroform followed by ethanol precipitation). The protocol takes place over 8 days.
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Radvan et al. (2025) studied this question.
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