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September 10, 2025The AnalystOpen Access

Assessment of IgG Stability in Low pH Elution Buffer using ATR-FTIR Spectroscopic Imaging and Microfluidics

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Authors

CHCéline van HaarenImperial College London BBBernadette ByrneImperial College London SKSergei G. KazarianImperial College London

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Implication

Investigation reveals low pH triggers aggregate formation in monoclonal antibodies, highlighting the role of ATR-FTIR and microfluidics in studying structural stability.

Key Points

Low pH environment significantly destabilizes IgG antibodies, leading to rapid aggregation at higher temperatures.
Under flow, structural changes in IgG were noted at both 30 °C and 45 °C, with major shifts in the Amide I peak indicative of beta sheet formation.
The study utilized ATR-FTIR spectroscopic imaging and microfluidics for non-destructive stability assessments, suggesting effectiveness under bioprocessing conditions.
Results underscore the importance of monitoring monoclonal antibody stability to mitigate potential immunogenic responses in patients.

Cite This Study

Haaren et al. (2025) studied this question.

synapsesocial.com/papers/68c1b61454b1d3bfb60eb51f https://doi.org/10.1039/d5an00664c

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Implication

Investigation reveals low pH triggers aggregate formation in monoclonal antibodies, highlighting the role of ATR-FTIR and microfluidics in studying structural stability.

Assessment of IgG Stability in Low pH Elution Buffer using ATR-FTIR Spectroscopic Imaging and Microfluidics

Discussion

Member takes

Implication

Key Points

Cite This Study

Assessment of IgG Stability in Low pH Elution Buffer using ATR-FTIR Spectroscopic Imaging and Microfluidics

Discussion

Member takes

Implication

Key Points

Cite This Study

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