This protocol facilitates direct DNA extraction from grapevine samples, enhancing high-throughput sequencing for phytoplasma analysis.
This protocol enables the direct enrichment of phytoplasma DNA from field samples, eliminating the need for propagation in test plants. It was developed using grapevine samples with low Flavescence dorée phytoplasma concentrations and incorporates both pre- and post-extraction enrichment steps to enhance the suitability of the extracted DNA for high-throughput sequencing (HTS) aimed at whole-genome analysis. Whole-genome amplification is an optional final step but is required when sequencing with Oxford Nanopore Technologies platforms.
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Zwitter et al. (2025) studied this question.
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