Lipid peroxidation and antioxidant status in 2,4,6-octatrienoic acid treated A549 and HCT-116 cancer cells

The thiobarbituric acid reactive substances (TBARS) and antioxidants status have been utilized as putative biomarkers to assess the in vitro antiproliferative effect of phytoconstituents and medicinal plants in cancer cells. This study analysed the status of lipid peroxidation and antioxidant status in untreated and octatrienoic acid treated A549 lung and HCT-116 colorectal cancer cells. The status of lipid peroxidation by-products [TBARS, Conjugated Dienes (CD) and lipid hydroperoxides] and activities of antioxidants [Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Reduced glutathione (GSH)] were analysed using colorimetric assays. While octatrienoic acid increased the TBARS, CD and lipid hydrperoxides levels, it lowered the activities of SOD, CAT, GPx and GSH content in A549 and HCT-116 cells. The results of present study revealed that octatrienoic acid might have suppressed the proliferation of A549 lung and HCT-116 colorectal cancer cells through modulating the oxidative stress biomarkers. The present study concludes that octatrienoic acid facilitates the generation of lipid peroxidation by-products by reducing antioxidant defence mechanism in A549 and HCT-116 cancer cells.